The fine genome of S. indicum L., var. Yuzhi 11(PRJNA74261) has been constructed using the complicated ‘Illumina + Roche 454 +BAC paired-end (ABI3730) + PacBio’ sequencing platform and the ‘SNP genetic map+ BAC-FISH physical map + Optical map’ assisted assembly method. Thefinal genome assembly reaches 335.9Mb, occupying 94.8% of the 354Mb genome estimatedby K-mer. In the fine genome map, the number of total scaffolds is 3,734, mostof which are distributed into the 13 chromosomes of sesame karyotype. Thenumber of N50 scaffolds is 18 and the N50 scaffold size is 6.38Mb. The numberof N90 scaffolds is 886 and the N90 scaffold size is 19.6Kb. The largestscaffold covers 14.1Mb. With the aid of ultra-density SNP genetic linkage mapcarrying 30,194 unique SNPs and the physical map harboring 210 BACs, the finegenome map for sesame was generated. In the sesame genome, a totalof 31,462 gene models are found and 17% are related to fatty acid synthesis andmetabolism. (Being prepared for publication)
2.The Ultra-density SNP Genetic Map of Sesame
We systematically screened the genome-wide SNPs of the F2 mapping population and constructed the ultra-density SNP genetic linkage map for sesame using next generation sequencing technique. A total of 13 linkage groups were in accordance with the 13 chromosomes in sesame karyotype. (Being prepared for publication)
3. The sesame choroplast genome
In order to investigate the evolutionary characters according to the Sesame Genome Project, apart from sequencing its nuclear genome, the complete chloroplast genome of S. indicum cv. Yuzhi 11 (white seeded) was sequenced using Illumina and 454 sequencing and assembled. Thechloroplast genome of cv. Yuzhi 11 contains 153,338 bp and a total of 114 unique genes (KC569603). (Having been published)