Sesame genome sequencing material--Yuzhi 11, a cultivar planted widely in China
Yuzhi 11, a cultivar planted widely in China was selected as sesame genome sequencing material. The genome size of the cultivar is estimated by flow cytometry to be about 369 Mb. Yuzhi 11, which represents S.indicum cultivars with a simple stem, three flowers per axilla, oblong-quadrangular capsules, and white flower and seed-coat color. Yuzhi 11 is one of the most important Chinese cultivars due to its high oil content (56.66%), resistance to fungal pathogens such as Fusarium wilt, Charcoal rot and Alternaria leaf spot, and waterlogging stress, and is cultivated in the main production regions of China.
Sesame Microsporogenesis Observation and Nulciec Sterility Mechanism
To investigate sesame flower development and nucleic sterility mechanism, the microspore development in sesame nucleic male sterile (NMS) line, MS86-1 was observed and compared using electron microscope techniques. Microsporogenesis of fertile, partial sterile and sterile plants differentiated with each other at the meiosis stage.
Analysis of Genetic Diversity and Population Structure of Germplasm Resources in Sesame
(Sesamum indicum L.)
The genetic diversity and population structure of 545 sesame accessions, including domestic and foreign germplasm resources were analyzed systematically with 42 SSR primer pairs. Sesame diversity capability seemed minor for the close genetic relationship among the worldwide sesame germplasm resources. The genetic diversity of domestic germplasm inChinawas not completely consistent with geopraphic distribution, and the genetic basis of main cultivars in China was narrow relatively. The introduction of foreign resources should be reinforced, while the domestic resources from different origins be utilized to widen the cultivars' genetic basis in the future sesame breeding.
Technical System of Plantlet Regeneration and transgenic plant generation in Sesame
The technical system of sesame callus tissue induction and shoot regeneration with high frequency has been established in 2010 (Miao et al., 2012). Among the growth regulators, BA and NAA are suitable for inducing compact green callus with a high differentiation frequency. The transgenic technique is formed using Agrobacterium tumefaciens mediated transfer method in 2011. 30 lines of transgenic sesame plants carrying exogenous GUS gene and Glucan gene are obtained from callus tissues, which have been screened by PCR and Southern blotting.